Abstract 

Sensory neuron membrane proteins (SNMPs) are widely distributed in parasitoid wasps and play a pivotal role in multiple essential activities including feeding, mating, egg laying, and host localizing. In this study, the AoSNMP1 of Anastatus orientalis Yang and Chou (Hymenoptera: Eupelmidae) was cloned by rapid amplification of complementary DNA (cDNA) ends based on the transcriptome data of A. orientalis. The nucleotide and deduced amino acid sequences of AoSNMP1 were analyzed by bioinformatics software. The bioinformatic analysis showed that the full-length cDNA of AoSNMP1 was 1,928 bp (GenBank accession PV545919), with a noncoding region of 211 and 121 bp at the 5′and 3′ end, respectively. AoSNMP1 contains a 1,596-bp open reading frame encoding 531 amino acids, with a molecular mass of 60.873 kDa and isoelectric point of 5.54. Phylogenetic analysis revealed that the AoSNMP1 of A. orientalis was closely related to the evolution of the SNMP1 in Nasonia vitripennis (Walker). Real-time quantitative polymerase chain reaction indicated that AoSNMP1 is expressed in both male and female adults as well as in various tissues. Notably, expression levels are significantly higher in female adults than in male adults. In addition, the expression level in the abdomen is markedly greater than that in both the head and thorax. This study provides a theoretical basis for further research on the function of AoSNMP1, especially on the olfactory receptive mechanism in A. orientalis.