Black soldier fly, Hermetia illucens (Diptera: Stratiomyidae), larvae are renowned for their bioconversion of organic waste into nutrient-rich supplements for various applications. In previous studies, the predominant genera of gut bacteria show a large variability among fly larvae, likely due to variability in diets. The ability of catabolic degradation by black soldier fly larvae might be ascribed to intestinal microorganisms. Diets can influence the gut microbiota of H. illucens. However, the effect of distinct foods on bacterial communities of gut bacteria is poorly understood. For this purpose, we undertook this study to assess the impacts of diet on the structure and function of the microbial communities in the gut of black soldier fly larvae fed with representative types of diets. We found that the most abundant bacteria in the black soldier fly larvae gut metagenome were Morganella (17.02%), Enterococcus (10.27%), Paenibacillus (9.50%), Klebsiella (7.29%), and Enterobacteriaceae (10.27%) and, thus, represent the core microbiome. Our results provide insights into the bacterial genes in H. illucens the larval gut, and we concluded that the microbiota structure and function could be shaped by the edible mushroom residue diet. Characterizing the interplay between the gut microbiome and black soldier fly larvae diets helps to clarify the underlying degradation processes and may contribute to improved large-scale black soldier fly larvae rearing. These data sets help to exploit the microbiological optimization of H. illucens as a sustainable insect for industrial rearing and the microbiome for novel biotechnological applications.Abstract
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